|Abstract: ||Lauraceae Cinnamomum genus is an important species of the broad-leaved evergreen, which is found in the subtropics. In Taiwan, it is often found in the low-elevated mountains, as sidewalk trees, or in campuses ... etc. During the earlier years, the central and southern region of Taiwan were even famous for its exporting of camphor. Due to Taiwan's unique climate, the Cinnamomum camphora plant species are considerably varied, which consisted mainly of camphor and cinnamon.
In this study, we used the headspace solid phase microextraction and gas chromatography-mass spectrometry to analyze the composition of volatiles from the leaves of linalool-type C. camphora (L.) Presl var, eucalyptol-type C.osmophloeum and borneol-type C. burmanni and the optically active component's optical purity.
First, in the 11 optically active standard temperature tests. At the temperature of 25ﾟC , the percentage of volatile areas were (-)-α-pinene 7.76%, (+)-α-pinene 4.67%, (+)-β-pinene 12.17%, (-)-β-pinene 12.52%, 14.78% eucalyptol. At the temperature of 40ﾟC, the percentage of volatile areas were (-)-linalool 6.71%, (+)-linalool 6.8%, camphor 26.67%, (-)-borneol 14.18%, (+)-borneol 19.2%. Using the seven different solid phases of micro extraction adsorb material tests, with which PDMS 100um analysis of the standard total area being the maximum. Followed by DVB / CAR / PDMS> PDMS / DVB> PA> PEG> PDMS 30um, and PDMS 7um appeared to have no signal.
HP-5MS and cyclodex-B, the two types of analytical columns, that were used to analyze all types of volatile components. The HP-5MS column analyzed the linalool-type C. camphora (L.) Presl var, which showed 24 components, eucalyptol-type C.osmophloeum showed 22 components, and borneol-type
C. burmanni showed 8 components. And the relatively cyclodex-B column analyzed the linalool-type C. camphora (L.) Presl var which showed 35 components, eucalyptol-type C.osmophloeum showed 42 components, and borneol-type
C. burmanni showed 21 components.
The cyclodex-B column analysed leaf's optical active isomer's main volatile components, linalool-type C. camphora (L.) Presl var was (-)-linalool, α-pinene enantiomeric had the purity of 72%, β-pinene enantiomeric had the purity of 65%；eucalyptol-type C.osmophloeum was (+)-borneol, α-pinene enantiomeric had the purity of 54%, β-pinene enantiomeric had the purity of 30%; borneol-type
C. burmanni was (+)-borneol, α-pinene enantiomeric had the purity of 55%, β-pinene enantiomeric had the purity of 17%. The differences in the optically active compositions were quite large, therefore, this study successfully provided methods to rapidly identify Cinnamomum.