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    题名: In human T cells mifepristone antagonizes glucocorticoid non-genomic rapid responses in terms of Na+/H+-exchange 1 activity, but not ezrin/radixin/moesin phosphorylation.
    作者: 賈愛華;Chien, Eileen Jea;*;許敬暉;Hsu, Ching-Hui;張瀚之;Chang, Vincent Han-Jhih;林品儀;Lin, Enoch Pin-Yi;郭品岑;Kuo, Trista Pin-Tsun;簡肇衡;Chien, Chau-Heng;林孝義;Lin, Hsiao-Yi;*
    贡献者: 健康產業管理學系
    日期: 2016-07
    上传时间: 2016-11-08 10:28:43 (UTC+8)
    摘要: Glucocorticoids (GCs) and progesterone have been employed as immunosuppressive agents during pregnancy for many years. Intracellular acidification by GCs is due to a rapid non-genomic inhibition of membrane Na+/H+-exchange 1 (NHE1) activity and is followed by immunosuppression of PHA-stimulated proliferation. NHE1 is tethered to the cortical actin cytoskeleton through ezrin/radixin/moesin (ERM) proteins within lipid rafts; these regulate cell shape, migration and resistance to apoptosis. We explored whether mifepristone (RU486), an antagonist of GCs in T cells, is able to completely block rapid non-genomic responses, namely NHE1 activity and the phosphorylation C-terminal residues of ERM proteins at threonine (cp-ERM).

    GCs stimulate a rapid non-genomic cp-ERM response in cells within 5 min. RU486 antagonized the GC-induced rapid decrease in NHE1 activity, and arrested PHA-stimulated T cells at G0/G1 phase but had no effect on the rapid increase in cp-ERM, which persisted for 24 h. However, the cp-ERM response was blocked by staurosporine in both resting and GC stimulated cells. The results of RU486 antagonized the GC induced rapid decrease in NHE1 ion transport activity, but not the increase cp-ERM. This suggests that RU486 in T cells exerts its antagonistic effects at NHE1 containing plasma membrane sites and not where cp-ERM links lipid rafts to cortical cytoskeletons.
    關聯: STEROIDS
    显示于类别:[健康產業管理學系] 期刊論文


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