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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/102141

    Title: Tetrandrine induces apoptosis Via caspase-8, -9, and -3 and poly (ADP ribose) polymerase dependent pathways and autophagy through beclin-1/ LC3-I, II signaling pathways in human oral cancer HSC-3 cells
    Authors: 余富順;Yu, Fu-Shun;Yu, Chun-Shu;Yu, Chun-Shu;Ch, Jaw-Chyun;Chen, Jaw-Chyun;Ya, Jiun-Long;Yang, Jiun-Long;Lu, Hsu-Feng;Lu, Hsu-Feng;Chan, Shu-Jen;Chang, Shu-Jen;Lin, Meng-Wei;Lin, Meng-Wei;鍾景光*
    Contributors: 生物科技學系
    Date: 2016-04
    Issue Date: 2017-03-01 14:05:36 (UTC+8)
    Abstract: Tetrandrine is a bisbenzylisoquinoline alkaloid that was found in the Radix Stephania tetrandra S Moore. It had been reported to induce cytotoxic effects on many human cancer cells. In this study, we investigated the cytotoxic effects of tetrandrine on human oral cancer HSC-3 cells in vitro. Treatments of HSC-3 cells with tetrandrine significantly decreased the percentage of viable cells through the induction of autophagy and apoptosis and these effects are in concentration-dependent manner. To define the mechanism underlying the cytotoxic effects of tetrandrine, we investigated the critical molecular events known to regulate the apoptotic and autophagic machinery. Tetrandrine induced chromatin condensation, internucleosomal DNA fragmentation, activation of caspases-3, -8, and -9, and cleavage of poly (ADP ribose) polymerase (PARP) that were associated with apoptosis, and it also enhanced the expression of LC3-I and -II that were associated with the induction of autophagy in human squamous carcinoma cell line (HSC-3) cells. Tetrandrine induced autophagy in HSC-3 cells was significantly attenuated by bafilomycin A1 (inhibitor of autophagy) pre-treatment that confirmed tetrandrine induced cell death may be associated with the autophagy. In conclusion, we suggest that tetrandrine induced cell death may be through the induction of apoptosis as well as autophagy in human oral cancer HSC-3 cells via PARP, caspases/Becline I/LC3-I/II signaling pathways. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 395–406, 2016.
    Appears in Collections:[生物資訊與醫學工程學系 ] 期刊論文

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