ASIA unversity:Item 310904400/107353
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 90452/105769 (86%)
造访人次 : 11930487      在线人数 : 444
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/107353


    题名: The Protocol for Dendrobium Officinale Mass Propagation
    作者: WU, YU-HSIEN
    贡献者: 生物科技學系
    关键词: Dendrobium officinale;Tissue culture
    日期: 2017
    上传时间: 2017-09-13 13:28:51 (UTC+8)
    出版者: 亞洲大學
    摘要: There are more than 1,600 species of Dendrobium in the world. Some of them had been reported can be used as medicine herb, for example D. officinale, D. huoshanense, D. moniliforme and D. nobile. Dendrobine is the alkaloids extract from Dendrobium spp. which can cure analgesic, antipyretic, reduce heart rate, high blood pressure and slow breathing. A ccording to previous research, D.officinale can increase NO and TNF-α increased macrophage secretion and phagocytosis to enhance the immune mechanism, it can also promote islet cells make insulin and glucagon, to achieve the effect of lowering blood pressure. Heavily harvested due to its medicinal effects, D. officinale is rarely can be seen in nature also markets appeared to be short in supply, and it also found out cannot year-round planting in Taiwan. The cultivation environment such as temperature tolerance of D. officinale is from 18℃ to 30℃, and it will stop growing at temperature more than 35℃. Therefore, the method to cultivate large amount of D. officinale is an important topics at present.
      The artificial reproduction of D. officinale was carried out by tissue culture and environment. The environment result shows D. officinale grow 0.64 cm/month in red + white light (3474 lux), the highest compare with other light intensities. A capsule of D. officinale germination can sprout 1713 seedling. In addition, the buds grow optimally in red light (1304 lux) which is 1 unit/24 days. the seeds are from capsule of D. officinale on MS agar for germination, than inoculation to seedling medium until adult. But the genetic will be changed by this method. In this research, we used high temperature treatment to induce D. officinale stem adventitious buds. The result shows the probability of inducing adventitious buds was 50.28%. The growth regulators also effect the growth of the D. officinale buds. The result showed 2ppm 2, 4-D + 0.5ppm 6-BA MS medium have the fastest growing, the growth rate is 0.026 cm/days. Using ½ MS agar without growth regulators is the most effective way to induce lateral buds, an average of 6 days can induce a bud. Combining all the data, it is possible to induce the buds for D. officinale in high temperature and propagation it in medium low temperature such as 25℃ in ½ MS agar without growth regulators.
    Combining all the data, 60,000 D. officinale can growth 2,239,470 in first year, then get 39,188,666 plants in second years. Through it is possible to cultivate the D. officinale in plant factory and have the economic benefits of the market.
    显示于类别:[生物科技學系] 博碩士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML83检视/开启


    在ASIAIR中所有的数据项都受到原著作权保护.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈