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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/108081

    Title: SARS coronavirus papain-like protease up-regulates the collagen expression through non-Samd TGF-β1 signaling
    Authors: 林振文;Ching-Ying W;Ching-Ying Wang;Chien-Yi Lu;Shih-Wen Li;Chien-Chen L;Chien-Chen Lai;Chun-Hung Hu;Chun-Hung Hua;黃素華;Su-Hua Huang;Ying-Ju Lin;Mann-Jen Hou;Mann-Jen Hour
    Contributors: 生物科技學系
    Date: 2017-05
    Issue Date: 2017-10-30 10:35:36 (UTC+8)
    Abstract: SARS coronavirus (CoV) papain-like protease (PLpro) reportedly induced the production of TGF-β1 through p38 MAPK/STAT3-meidated Egr-1-dependent activation (Sci. Rep. 6, 25754). This study investigated the correlation of PLpro-induced TGF-β1 with the expression of Type I collagen in human lung epithelial cells and mouse pulmonary tissues. Specific inhibitors for TGF-βRI, p38 MAPK, MEK, and STAT3 proved that SARS-CoV PLpro induced TGF-β1-dependent up-regulation of Type I collagen in vitro and in vivo. Subcellular localization analysis of SMAD3 and SMAD7 indicated that non-SMAD pathways in TGF-β1 signaling involved in the production of Type I collagen in transfected cells with pSARS-PLpro. Comprehensive analysis of ubiquitin-conjugated proteins using immunoprecipitation and nanoLC–MS/MS indicated that SARS-CoV PLpro caused the change in the ubiquitination profile of Rho GTPase family proteins, in which linked with the increase of Rho-like GTPase family proteins. Moreover, selective inhibitors TGF-βRI and STAT6 (AS1517499) ascertained that STAT6 activation was required for PLpro-induced TGF-β1-dependent up-regulation of Type I collagen in human lung epithelial cells. The results showed that SARS-CoV PLpro stimulated TGF-β1-dependent expression of Type I collagen via activating STAT6 pathway.
    Relation: VIRUS RESEARCH
    Appears in Collections:[生物科技學系] 期刊論文

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