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    题名: Study on the inhibitory effect of Scutellaria baicalensis Gergi on neuraminidase of influenza A virus
    作者: Yen-Kuan Lin
    贡献者: Department of Biotechnology
    关键词: Scutellaria baicalensis Gergi;neuraminidase;influenza
    日期: 2010
    上传时间: 2010-11-08 09:10:50 (UTC+8)
    出版者: Asia University
    摘要: Scutellaria baicalensis Georgi is often included as an ingredient in traditional Chinese compound prescriptions for the treatment of fever-related or inflammatory conditions. It has been demonstrated to have various clinical activities, including anti-virus, anti-allergic, anti-inflammatory, anti-microbial, and anti-cancer effects. Influenza A virus (IAV) belongs to the Orthomyxovirus family, being segmented, single stranded, negative sense RNA virus. IAV is the important respiratory pathoge, being responsible for both seasonal influenza outbreaks and periodic world-wide pandemics. The goal of this study is to investigate the antiviral effect of Scutellaria baicalensis Gergi and their marker compounds against Influenza A virus (IAV) in vitro assay. First, neuraminidase inhibition activity assay was used to measure for 50% inhibiting concentration (IC50) of each extract and marker compounds. The MTT assay was used to measure for 50% cytotoxicity concentration (CC50) of each extract and marker compounds. To investigate the efficacy of Scutellaria baicalensis Gergi and their marker compounds as antivirals against Influenza A virus by assessing their ability to reduce Influenza A virus RNA levels in MDCK cells. The plaque assay was carried out detecting IC50 of extracts and compounds of Scutellaria baicalensis Gergi. The IC50 values of methanol extract, ethyl acetate extract, chloroform extract, baicalein, baicalin, apigenin and chrysin were 473.93 μg/ml、 57.14 μg/ml、103.51 μg/ml、0.423 μM、 2.55 μM、61.72 μM and 109.64 μM for in vitro neuraminidase inhibition activity assay. Moreover, Scutellaria baicalensis Gergi and its marker compounds inhibited the neuraminidase activity of IAV. Extracts and compounds of Scutellaria baicalensis Gergi showed the inhibitory ability on the IAV-induced CPE in MDCK cells. Otherwise, real-time PCR demonstrated the inhibition of IAV titer by extracts and the marker compounds of Scutellaria baicalensis Gergi. Therefore we concluded that Scutellaria baicalensis Gergi is effective in inhibiting the replication of influenza virus, and it is more likely to be a potential antiinfluenza viral agent for clinical use.
    显示于类别:[生物科技學系] 博碩士論文

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