Having a balanced diet is important to middle school students who are growing. Research has shown that middle high school students have the problem of having unbalanced diet, especially the insufficient intake in vegetables and fruits. The health and nutrition of the middle school students has been one of the prioritized issues of the World Health Organization (WHO). WHO has developed a world-wide surveillance system, namely, the Global Students Heath Survey (GSHS) to watch the students’ health behaviors and conditions? Fruit and vegetable intake is one of the key issues to be investigated in the survey. The purpose of this study was to determine the fruit and vegetable intake and the relationship between vegetable and fruit intake and other health related behaviors and conditions of the middle school students in Taichung County and Taichung City to provide information for educational and and other resources allocation for policy makers in the future. This study used a cross-sectional study design and adeopted the GSHS survey questionnaire to examine middle school students’ health related behavior. The population studied was the jounior high school students of Taichung County and Taichung City registrated in the year of 2010. The questionnaire survey was conducted during the time of June 2010. A total of 607 students answered and returned the questionnaire for this analysis. The statistical methods adopted for the data analysis are Spearman correlation analysis, Chi-square test and Binary Logistic Regression. The main results are as followings: 1. About 40% of the students reported having vegetable 3 times or more a day; 20% of the students reported having fruit 2 times or more a day. 2. Students from Taichung County were more likely to have insufficient fruit intake compared with that of the students from Taichung City. No significant difference in vegetable intake has been found between the students from the city and the county. 3. Positive associations have been found between personal health practice, family protection factors and vegetable and fruit intake; negative associations have been found between grade level, physical activities and vegetable and fruit intake. Schools and families should make the efforts on the accessibility of vegetable and fruit intake and emphasize on the importance of fruit and vegetable consumption. Providing more vegetable and fruit in school lunch and providing effective food and nutrition education for the students is recommended.