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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/110725


    Title: Phenethyl Isothiocyanate (PEITC) and Benzyl Isothiocyanate (BITC) Inhibit Human Melanoma A375.S2 Cell Migration and Invasion by Affecting MAPK Signaling Pathway In Vitro
    Authors: MA, YI-SHIH;HS, YUNG-TING;HSIAO, YUNG-TING;LIN, JEN-JYH;CHING-LUNG, LUNG L;LIAO, CHING-LUNG;CHIN-CHUNG, L;LIN, CHIN-CHUNG
    Contributors: 生物科技學系
    Date: 2017-11
    Issue Date: 2018-04-03 09:14:31 (UTC+8)
    Abstract: Background/Aim: Numerous evidence has shown
    that PEITC and BITC inhibit cancer cell migration and
    invasion. In this study, we investigated the anti-metastatic
    mechanisms of PEITC and BITC in human melanoma cancer
    A375.S2 cells in vitro. Materials and Methods: We used a
    cell viability assay, an in-vitro scratch wound healing assay,
    a transwell assay for cell migration and invasion, a gelatin
    zymography assay, western blotting and EMSA to examine
    the anti-metastatic mechanisms of PEITC and BITC in
    A375.S2 cells. Results: Sublethal concentrations of PEITC
    (0, 1, 2 and 2.5 μM) and BITC (0, 0.5, 1 and 2 μM) inhibited
    mobility, migration and invasion of A375.S2 cells that were
    assayed by wound healing and Transwell filter. PEITC and
    BITC inhibited MMP-2 activity in A375.S2 cells, as assessed
    by gelatin zymography assay. Results from western blotting
    indicated that PEITC (2.5 μM) and BITC (2 μM) decreased
    the levels of p-p38 following 24 and 48 h treatment. PEITC
    (1-2.5 μM) reduced the levels of p-JNK1/2 proteins following
    48-h treatment but BITC increased p-JNK1/2 levels following
    24-h treatment. PEITC (2.5 μM) reduced the levels of pERK1/2
    proteins following 48-h treatment but BITC (0.5-2
    μM) increased p-ERK1/2 levels following 24- and 48-h
    treatment. PEITC and BITC affect cell migration and
    invasion of A375.S2 cells via MAPK pathway. PEITC and
    BITC inhibited MMP-2 activity. PEITC increased NF-ĸB
    expression but BITC decreased NF-ĸB expression in the
    nucleus. Furthermore, NF-ĸB p65 binding to DNA was
    decreased following 2.5 μM PEITC treatment, but increased
    following treatment with 1-2 μM. However, 0.5-2 μM BITC
    treatment decreased the binding of NF-ĸB to DNA in
    A375.S2 cells, as assessed by electrophoretic mobility shift
    (EMSA) assay. Conclusion: Based on these observations, we
    suggest that PEITC and BITC can be used as antimetastastic
    agents of human melanoma cells in the future.
    Relation: ANTICANCER RESEARCH
    Appears in Collections:[生物科技學系] 期刊論文

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