The purpose of this study was to examine the effect of pickleball on the health related physical fitness of seniors. A total of 14 seniors with Taichung Daii Dist Lide Community aged from 60 to 75 were recruited. By means of random assignment subjects were divided into the experimental and control group with each 7 members. The experimental group excepted rope pickleball training conducted for 90 minutes, 3 times weekly for continually 10 weeks, the control group maintained the regular activities. All participants were examined with health related physical fitness before and after training. The analysis was conducted by dependent samples t-test and analysis of covariance (ANCOVA) and the results were listed as below: (A) Dependent samples t-test was used to analyze the difference of both groups between pre- and post- training. There was significant difference between flexibility and muscular endurance and reaction time and aerobic capacity(p＜.05). (B)Both groups showed significant differences between flexibility and muscular endurance and reaction time and aerobic capacity analyzed with ANCOVA (p＜.05). Conclusion: Rope pickleball had significant effect on the improvement of seniors in flexibility and muscular endurance and reaction time and aerobic capacity.