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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/11127


    Title: 舞菇T2品系乙酸乙酯萃取層之生物活性成份分析
    Authors: 方苡藻
    Contributors: Department of Biotechnology
    Keywords: 舞菇;超音波萃取;DPPH自由基清除能力;還原力測定;亞鐵離子螯合能力;高效能液相層析質譜儀;酪胺酸?
    Date: 2011
    Issue Date: 2011-09-09 16:57:18 (UTC+8)
    Publisher: Asia University
    Abstract: 本研究主要分析環控技術所栽培之舞菇(Grifola frondosa)所含之生物活性成份。以正己烷、乙酸乙酯與50%酒精分別進行超音波萃取乾燥粉末樣品,其萃取率分別為1.60 ± 0.36%、3.10 ± 0.17%與22.90 ± 0.40%。測試不同粗萃物之DPPH自由基清除能力、還原力與亞鐵離子螯合能力,結果顯示粗萃物於三種抗氧化測試中皆有良好效果。利用高效能液相層析質譜儀(High-performance Liquid Chromatography-Mass Spectrophotometer)檢測各萃取層內之成份,於乙酸乙酯萃取層與正己烷萃取層共測得7個分離峰,取其分離峰相對純化物進行三種抗氧化能力測試,結果顯示S4與S5具有清除DPPH自由基的能力(EC90各為0.96 ± 0.02mg/ml及0.04 ± 0.02mg/ml),以S5清除自由基的能力最好;還原力測定結果顯示S4、S5均具有還原力,S4之還原力約為BHT的36.08 ± 1.36%,S5之還原力則高於BHT(>100%);亞鐵離子螯合能力的測試結果,則發現五種成份並不具有亞鐵離子螯合能力;經由酪胺酸?的測試則推斷其抑制型態為不競爭性抑制。
    Appears in Collections:[生物科技學系] 博碩士論文

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