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http://asiair.asia.edu.tw/ir/handle/310904400/111604
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| Title: | Potential role of CBX7 in regulating pluripotency of adult human pluripotentlike olfactory stem cells in stroke model |
| Authors: | Fan,Jia-Rong;Fan,Jia-Rong;Lee,Hsu-Tung;Lee,Hsu-Tung;Lee,Wei;Lee,Wei;Li,Chen-Huan;Lin1,Chen-Huan;Hsu,Chun Y.;Hsu,Chun Y.;Hs,Chia-Hung;Hsieh,Chia-Hung;徐偉成;SHYU,WOEI-CHERNG* |
| Contributors: | 職能治療學系 |
| Date: | 2018-05 |
| Issue Date: | 2018-10-22 11:48:46 (UTC+8) |
| Abstract: | The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopulation of adult pluripotent-like olfactory stem cells (APOSCs), which were modulated by an epigenetic repressor of CBX7. APOSCs form a floating sphere, express pluripotency markers Nanog, Oct-4, Sox-2, and SSEA-4 and show alkaline phosphatase activity. In addition, APOSCs display self-renewal and a pluripotent potential to differentiate into all three germ layers. Moreover, APOSCs coexpress pluripotency markers with CBX7. Within their natural niche, APOSCs from CBX7+/+ mice responded promptly to either spontaneous or injury-induced tissue regeneration. However, APOSCs from CBX7−/− mice manifested an impaired self-renewal and differentiation potential. Similarly, in vitro-cultivated CBX7−/− APOSCs underwent premature senescence, whereas CBX7+/+ APOSCs still actively divided, indicating that CBX7 is required for the self-renewal of APOSCs. Intracerebral implantation of APOSCs improved the stroke-mediated neurological dysfunction in rodents. These findings indicate that CBX7 plays a critical role in the regenerative properties of APOSCs and indicate the safety and feasibility of implantation of autologous APOSCs in stroke treatment. |
| Relation: | Cell Death & Disease |
| Appears in Collections: | [職能治療學系] 期刊論文
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