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|Title: ||Potential role of CBX7 in regulating pluripotency of adult human pluripotentlike olfactory stem cells in stroke model|
|Authors: ||Fan,Jia-Rong;Fan,Jia-Rong;Lee,Hsu-Tung;Lee,Hsu-Tung;Lee,Wei;Lee,Wei;Li,Chen-Huan;Lin1,Chen-Huan;Hsu,Chun Y.;Hsu,Chun Y.;Hs,Chia-Hung;Hsieh,Chia-Hung;徐偉成;SHYU,WOEI-CHERNG*|
|Issue Date: ||2018-10-22 11:48:46 (UTC+8)|
|Abstract: ||The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopulation of adult pluripotent-like olfactory stem cells (APOSCs), which were modulated by an epigenetic repressor of CBX7. APOSCs form a floating sphere, express pluripotency markers Nanog, Oct-4, Sox-2, and SSEA-4 and show alkaline phosphatase activity. In addition, APOSCs display self-renewal and a pluripotent potential to differentiate into all three germ layers. Moreover, APOSCs coexpress pluripotency markers with CBX7. Within their natural niche, APOSCs from CBX7+/+ mice responded promptly to either spontaneous or injury-induced tissue regeneration. However, APOSCs from CBX7−/− mice manifested an impaired self-renewal and differentiation potential. Similarly, in vitro-cultivated CBX7−/− APOSCs underwent premature senescence, whereas CBX7+/+ APOSCs still actively divided, indicating that CBX7 is required for the self-renewal of APOSCs. Intracerebral implantation of APOSCs improved the stroke-mediated neurological dysfunction in rodents. These findings indicate that CBX7 plays a critical role in the regenerative properties of APOSCs and indicate the safety and feasibility of implantation of autologous APOSCs in stroke treatment.|
|Relation: ||Cell Death & Disease|
|Appears in Collections:||[職能治療學系] 期刊論文|
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