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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/112060


    Title: The C-Terminus of Hepatitis B Virus-encoded X Protein Is Required for Lapatinib Sensitivity in Hepatocellular Carcinoma Cells
    Authors: 陳貞妤;CHEN, JHEN-YU;黃偉謙;Huang, Wei-Chien;CHING-TING, W;WEI, CHING-TING;CH, PEI-HSUAN;CHIEN, PEI-HSUAN;陳韻如;CHEN, YUN-JU
    Contributors: 生物科技學系
    Date: 2019-02
    Issue Date: 2019-09-18 11:00:19 (UTC+8)
    Abstract: BACKGROUND/AIM:
    Hepatitis B virus-encoded X protein (HBx) plays a pivotal role in hepatocellular carcinoma (HCC) progression and treatment resistance. Interestingly, our previous study unexpectedly showed that full-length HBx sensitized HCC cells to lapatinib by up-regulating erb-b2 receptor tyrosine kinase 3 (ERBB3). We further aimed to map the exact motif within the HBx sequence responsible for lapatinib sensitization.

    MATERIALS AND METHODS:
    The exact motif responsible for the lapatinib sensitization was assessed by construction of various fragments of HBx. Cell viability was examined by the MTT assay and crystal violet staining.

    RESULTS:
    Our investigation found that lapatinib sensitivity and up-regulation of ERBB3 promoter activity were observed only in HCC cells expressing C-terminal residues of HBx. Furthermore, C-terminal HBx peptide induced ERBB3 protein expression and sensitivity to lapatinib.

    CONCLUSION:
    These results not only indicate that the C-terminus of HBx is required for lapatinib sensitivity, but also provide clues to developing a predictive biomarker for response of HCC to lapatinib in the future.

    Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
    Relation: ANTICANCER RESEARCH
    Appears in Collections:[生物科技學系] 期刊論文

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