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|Title: ||3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration|
|Authors: ||And, Yuan-Haw;Wu, Yuan-Haw Andrew;Yung-Cheng, Cheng C;Chiu, Yung-Cheng;Lin, Yen-Hong;Lin, Yen-Hong;Ho, Chia-Che;Ho, Chia-Che;謝明佑;Shie, Ming-You;陳怡文;Chen, Yi-Wen|
|Issue Date: ||2019-09-18 11:11:39 (UTC+8)|
|Abstract: ||Currently, clinically available orthopedic implants are extremely biocompatible but they lack specific biological characteristics that allow for further interaction with surrounding tissues. The extracellular matrix (ECM)-coated scaffolds have received considerable interest for bone regeneration due to their ability in upregulating regenerative cellular behaviors. This study delves into the designing and fabrication of three-dimensional (3D)-printed scaffolds that were made out of calcium silicate (CS), polycaprolactone (PCL), and decellularized ECM (dECM) from MG63 cells, generating a promising bone tissue engineering strategy that revolves around the concept of enhancing osteogenesis by creating an osteoinductive microenvironment with osteogenesis-promoting dECM. We cultured MG63 on scaffolds to obtain a dECM-coated CS/PCL scaffold and further studied the biological performance of the dECM hybrid scaffolds. The results indicated that the dECM-coated CS/PCL scaffolds exhibited excellent biocompatibility and effectively enhanced cellular adhesion, proliferation, and differentiation of human Wharton’s Jelly mesenchymal stem cells by increasing the expression of osteogenic-related genes. They also presented anti-inflammatory characteristics by showing a decrease in the expression of tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1). Histological analysis of in vivo experiments presented excellent bone regenerative capabilities of the dECM-coated scaffold. Overall, our work presented a promising technique for producing bioscaffolds that can augment bone tissue regeneration in numerous aspects.
Keywords: calcium silicate, polycaprolactone, 3D scaffold, decellularized, extracellular matrix, osteogenesis
|Relation: ||INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES|
|Appears in Collections:||[生物資訊與醫學工程學系 ] 期刊論文|
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