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|Title: ||Anticancer activities of chalcone flavokawain B from Alpinia pricei Hayata in human lung adenocarcinoma (A549) cells via induction of reactive oxygen species?mediated apoptotic and autophagic cell death|
|Authors: ||許游章;Hseu, You-Cheng;Huang, Yu?Chi;Huang, Yu?Chi;Varadharajan;Thiyagarajan, Varadharajan;Chacko, Dony;Mathew, Dony Chacko;Lin, Kai?Yuan;Lin, Kai?Yuan;Ch, Ssu?Ching;Chen, Ssu?Ching;Liu, Jer?Yuh;Liu, Jer?Yuh;Hsu, Li?Sung;Hsu, Li?Sung;Li, Mei?Ling;Li, Mei?Ling;Ya, Hsin?Ling;Yang, Hsin?Ling|
|Issue Date: ||2019-09-18 11:36:54 (UTC+8)|
|Abstract: ||Chalcones found in fruits and vegetables have promising cancer chemopreventive properties. This study attempts to identify the anticancer efficacies of chalcone flavokawain B (FKB) in the rhizomes of Alpinia pricei Hayata by examining key molecular events in non-small-cell lung cancer (A549) cells. Our results indicated that in human A549 cells, FKB (0-15 μg/ml) decreases cell viability and colony formation, dysregulates the Bax: B-cell lymphoma 2 ratio and increases apoptotic DNA fragmentation. Mitochondrial (caspase-9/-3 and poly ADP ribose polymerase [PARP]) signaling was found to be involved in FKB-induced apoptosis. In addition, FKB-induced reactive oxygen species (ROS) generation, and N-acetylcysteine attenuated FKB-induced apoptotic cell death. Moreover, FKB triggered autophagy, as evidenced by the improved acidic vesicular organelle formation, lipidated light chain 3 (microtubule-related light chain 3) accumulation, and ATG7 expression and the decreased mammalian target of rapamycin phosphorylation. Furthermore, FKB suppressed ROS-mediated ATG4B expression. Inhibiting autophagy using 3-methyladenine/chloroquine diminished FKB-induced cell death, indicating that autophagy is triggered as a death mechanism by FKB. In summary, FKB has a crucial role in the execution and propagation of ROS-mediated apoptotic and autophagic cell death of lung adenocarcinoma cells. K E Y W O R D S Alpinia pricei hayata, apoptosis, autophagy, chalcone flavokawain B, ROS|
|Relation: ||JOURNAL OF CELLULAR PHYSIOLOGY|
|Appears in Collections:||[食品營養與保健生技學系] 期刊論文|
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