The development of softball sports originated in the 1970s, after winning the World Cup women's softball game, and won excellent results. In 2002, he won the bronze medal of the World Cup Softball Championship and the silver medal of the first Asian Games in the past year. It is also the highest score in the softball sports. Brilliant once. As the development of sports science is changing with each passing day, the use of various data collection methods and information technology to enhance the information of competitors before the game and assist sports performance is an essential condition for current combat operations. A perfect and wonderful official sports event, there is an indispensable basic role, one is the player in the game, therefore, the player is the most important role in the game, but the audience ignores another role, "the referee His duties are the law enforcers, managers, and service providers. The referee uses the spirit of the rules to strictly enforce the two teams to have a fair and just game, so that the game is brilliant, orderly, and stimulating. The purpose of this paper is to explore the influence of pressure source, work pressure and fatigue of softball referee. The analysis results show that physical injury has a negative influence on subjective fatigue experience, and it is not competent to have positive influence on work motivation, and control ability is not good for work. Motivation has a positive influence. Incompatibility is not good. Negative influence on attention is established. Poor control ability has a negative impact on attention. Physical injury has a positive influence on attention. Conflict has attention. The positive influence is established, and the inability to compete with the physical injury has a positive influence on the attention. The subjective fatigue experience has a positive influence on the fatigue, and the work motivation has a positive influence on the fatigue. The attention has positive effects on the fatigue. As the impact is established, the activity has a positive impact on fatigue.