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    ASIA unversity > 資訊學院 > 光電與通訊學系 > 期刊論文 >  Item 310904400/112858


    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/112858


    Title: Inhibiting the LPS-induced enhancement of mEPSC frequency in superficial dorsal horn neurons may serve as an electrophysiological model for alleviating pain
    Authors: Chin-Tsang, Y;Yang, Chin-Tsang;Hung, Shih-Ya;Hung, Shih-Ya;Sheng-Feng, H;Hsu, Sheng-Feng;MacDona, Iona;MacDonald, Iona;Jaung-Geng, L;Lin, Jaung-Geng;Lu, Sih-Ting;Luo, Sih-Ting;陳易宏;Chen, Yi-Hung
    Contributors: 光電與通訊學系
    Date: 2019-11
    Issue Date: 2020-09-01 14:18:52 (UTC+8)
    Publisher: 亞洲大學
    Abstract: Pain is a major primary health care problem. Emerging studies show that inhibition of spinal microglial activation reduces pain. However, the precise mechanisms by which microglial activation contributes to nociceptive synaptic transmission remain unclear. In this study, we measured spontaneous synaptic activity of miniature excitatory postsynaptic currents (mEPSCs) in rat spinal cord superficial dorsal horn (SDH, laminae I and II) neurons. Lipopolysaccharide (LPS) and adenosine triphosphate (ATP) increased the frequency, but not amplitude, of mEPSCs in SDH neurons. Microglial inhibitors minocycline and paeonol, as well as an astrocyte inhibitor, a P2Y1 receptor (P2Y1R) antagonist, and a metabotropic glutamate receptor 5 (mGluR5) antagonist, all prevented LPS-induced enhancement of mEPSC frequency. In mouse behavioral testing, minocycline and paeonol effectively reduced acetic acid-induced writhing and LPS-induced hyperalgesia. These results indicate that LPS-activated microglia release ATP, which stimulates astrocyte P2Y1Rs to release glutamate, triggering presynaptic mGluR5 receptors and increasing presynaptic glutamate release, leading to an increase in mEPSC frequency and enhancement of nociceptive transmission in SDH neurons. We propose that these effects can serve as a new electrophysiological model for evaluating pain. Moreover, we predict that pharmacologic agents capable of inhibiting the LPS-induced enhancement of mEPSC frequency in SDH neurons will have analgesic effects.
    Relation: Scientific Reports
    Appears in Collections:[光電與通訊學系] 期刊論文

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