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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/112963


    Title: Sphingosine-1-phosphate promotes PDGF-dependent endothelial progenitor cell angiogenesis in human chondrosarcoma cells
    Authors: Wan, Chao-Qun;Wang, Chao-Qun;Li, Chih-Yang;Lin, Chih-Yang;黃元勵;HUANG, YUAN-LI;Wan, Shih-Wei;Wang, Shih-Wei;Wang, Yan;Wang, Yan;Huang, Bi-Fei;Huang, Bi-Fei;Lai, Yu-Wei;Lai, Yu-Wei;We, Shun-Long;Weng, Shun-Long;Fong, Yi-Chin;Fong, Yi-Chin;湯智昕;Chih-Hsin, Tang
    Contributors: 醫學檢驗暨生物技術學系
    Keywords: S1P;PDGF-A;chondrosarcoma;angiogenesis;EPCs
    Date: 2019-12
    Issue Date: 2020-09-04 15:21:08 (UTC+8)
    Publisher: 亞洲大學
    Abstract: The malignant bone tumors that are categorized as chondrosarcomas display a high potential for metastasis in late-stage disease. Higher-grade chondrosarcomas contain higher levels of expression of platelet-derived growth factor (PDGF) and its receptor. The phosphorylation of sphingosine by sphingosine kinase enzymes SphK1 and SphK2 generates sphingosine-1-phosphate (S1P), which inhibits human chondrosarcoma cell migration, while SphK1 overexpression suppresses lung metastasis of chondrosarcoma. We sought to determine whether S1P mediates levels of PDGF-A expression and angiogenesis in chondrosarcoma. Surprisingly, our investigations found that treatment of chondrosarcoma cells with S1P and transfecting them with SphK1 cDNA increased PDGF-A expression and induced angiogenesis of endothelial progenitor cells (EPCs). Ras, Raf, MEK, ERK and AP-1 inhibitors and their small interfering RNAs (siRNAs) inhibited S1P-induced PDGF-A expression and EPC angiogenesis. Our results indicate that S1P promotes the expression of PDGF-A in chondrosarcoma via the Ras/Raf/MEK/ERK/AP-1 signaling cascade and stimulates EPC angiogenesis.
    Relation: Aging-US
    Appears in Collections:[Department of Medical Laboratory Science and Biotechnology] Journal Article

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