This study was initiated to evaluate the tyrosinase inhibitory effects and antioxidant activities (including DPPH radical scavenging , reducing power and ferrous ion chelating power) on the fruiting body extracts of Pleurotus eryngii using solvents under atmosphere and high pressure conditions. The results showed all extracts with antioxidant activities and tyrosinase inhibitory effects under a mg/ml level, and the efficiencies increased with increasing concentrations either under normal or high pressure conditions. The extracts using ethanol as sovent showed the better results than hot water. The fruiting body extracts of Pleurotus eryngii were incorporated into lipid nanoparticles for the further applications. The encapsulated lipid nanoparticles were produced by high pressure homogenizer using Caprylic / Capric Triglyceride and Glyceryl Monostearate as lipids. The optimization of the preparation conditions of the encapsulated nanoparticles was also discussed to increasing the entrappment efficiency. The lipid nanoparticle entrapped with the extracts of Pleurotus eryngii was prepared to 81% entrappment efficiency. The particle size of the lipid nanoparticle was about 80nm.