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    题名: Effects of fenvalerate on progesterone production in cultured rat granulose cells.
    作者: 張竣維;Hebron, C.Chang
    贡献者: 生物科技學系
    关键词: Fenvalerate;Steroidogenesis;Steroidogenic enzymes;Cultured rat granulosa cells
    日期: 2005
    上传时间: 2012-11-23 17:08:17 (UTC+8)
    摘要: In this study, primary serum-free cultured rat granulosa cells (rGCs) were used as a cellular model to investigate the effects of fenvalerate on progesterone production. Various concentrations (0, 1, 5, 25, 125 and 625 microM) of fenvalerate were added to the cell cultures for 24 h. rGCs were stimulated by compounds such as follicle-stimulating hormone (FSH), 8-bromo-cAMP or 22(R)-hydroxycholesterol (22R-HC). Progesterone production and intracellular cAMP content were measured in control and treated groups. Expression of P450 side chain cleavage enzyme (P450scc) and steroidogenic acute regulatory protein (StAR) were monitored by real-time PCR and Western blotting. Results showed that fenvalerate inhibited basal progesterone production in rGCs in the absence of stimulators. This inhibition was stronger in the presence of FSH and was not fully reversed by 8-bromo-cAMP or 22R-HC. The increase of cAMP content, stimulated by FSH, was inhibited by fenvalerate implicating that the intracellular cAMP-dependent signal pathway was involved. Fenvalerate reduced mRNA and protein expression of P450scc. These results suggested that multi-site inhibition of progesterone production by fenvalerate including a cAMP-dependent protein kinase pathway and reduction on P450scc gene expression and/or its enzymatic activity in rGCs.
    關聯: REPRODUCTIVE TOXICOLOGY;20(2):195-202.
    显示于类别:[生物科技學系] 期刊論文


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