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http://asiair.asia.edu.tw/ir/handle/310904400/16098
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| Title: | Anion exchanger inhibitor DIDS induces human poorly-differentiated malignant hepatocellular carcinoma HA22T cell |
| Authors: | Liu, Chung-Jung;Hwang, Jin-Ming;Wu, Trang-Tiau;Hsieh, Yi-Hsien;Wu, Cheng-Chung;Hsieh, Yih-Shou;Tsai, Chang-Hai;Wu, Hsi-Chin;黃志揚;HUANG, CHIH-YANG;Liu, Jer-Yuh |
| Contributors: | 生物科技學系 |
| Keywords: | HA22T;hepatocellular;carcinoma;cells Anion;exchanger 2 DIDS;Apoptosis Proliferation;Anion transport;activity |
| Date: | 2008 |
| Issue Date: | 2012-11-23 17:08:31 (UTC+8) |
| Abstract: | Anion exchangers (AEs) of the Cl-/HCO3- exchanger family contribute to the regulation of intracellular acid-base balance. Recently, we found that anion exchanger 2 (AE2) was significantly expressed in human hepatocellular carcinoma (HCC) and in poorly-differentiated human HCC HA22T/VGH cells. In the present study, we further explored the pharmacological function of AE in four human HCC cell lines (SK-Hep-1, HA22T/VGH, HepG2, and Hep3B) following the treatment of 4,4’-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS), an AEs specific inhibitor. After administrations with 400–1000 μM of DIDS, cell proliferation was greatly inhibited in a dose-dependent manner from 47.5 to 65.0% in higher malignant HA22T/VGH cells, but not in other cell lines. The results of 4,6-diamidino-2-phenylindole (DAPI) staining, DNA fragmentation and flow cytometric analysis further revealed that cell apoptosis induced by DIDS was also observed in HA22T/VGH cells. Therefore, these findings suggested that AE may be involved, in part, in the proliferation and survival of HA22T cells and could be a new potential therapeutic target against specific human HCC. |
| Relation: | MOLECULAR AND CELLULAR BIOCHEMISTRY |
| Appears in Collections: | [生物科技學系] 期刊論文
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