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|Title: ||Proliferative Effects of Chishao on Schwann Cells are FGF-uPA, and ERK- and JNK-Dependent|
|Authors: ||Huang, Kun Shan;Lu, Min-Jin;Chen, Yueh-Sheng;Tsai, Fuu-Jen;Kuo, Wei-Wen;Cheng, Yi-Chang;Lin, Chien-Chung;Tsai, Chang-Hai;黃志揚;HUANG, CHIH-YANG;Lin, Jaung-Geng;Ou, Hsiu-Chung|
|Keywords: ||Chishao;Proliferative Effect;Schwann Cells;Nerve Regeneration|
|Issue Date: ||2012-11-23 17:08:56 (UTC+8)|
|Abstract: ||"This study evaluated the proliferative effects of chishao on RSC96, Schwann cells. A dose-dependent proliferative effect of chishao was obtained by methylthiazol tetrazolium (MTT), proliferating cell nuclear antigen (PCNA) Western blotting, and wound healing assays in Schwann cells administered with chishao (0–500 mg/ml), except at 500 mg/ml concentration. The chishao-treated cells also showed a dose-dependent activated fibroblast growth factor-2 (FGF-2) signaling with increased urokinase plasminogen activator (uPA) and decreased plasminogen activator inhibitor-1 (PAI-1), enhanced proliferative proteins, extracellular signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK)-signaling. Using mitogen-actvated protein kinase (MAPK)-signaling chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of chishao on RSC cells were identified to be ERK- and JNK- signaling dependent. Based on the results, applying appropriate doses of chishao to Schwann cells would be a potential approach for enhancing neuron regeneration.[ABSTRACT FROM AUTHOR]
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|Relation: ||AMERICAN JOURNAL OF CHINESE MEDICINE|
|Appears in Collections:||[生物科技學系] 期刊論文|
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