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http://asiair.asia.edu.tw/ir/handle/310904400/16135
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| Title: | The Proliferate and Migration Effects of Huangqi On RSC96 Schwann Cells |
| Authors: | Ko, Fu-Yang;Wang, Hwai Lee;Kuo, Chia-Hua;Chen, Li-Mien;Tsai, Fuu-Jen;Tsai, Chang-Hai;Chen, Yueh-Sheng;Kuo, Wei-Wen;黃志揚;HUANG, CHIH-YANG |
| Contributors: | 生物科技學系 |
| Keywords: | Peripheral Nerve Regeneration;Proliferative Effect;Schwann Cells;Sciatic Nerve |
| Date: | 2009 |
| Issue Date: | 2012-11-23 17:09:00 (UTC+8) |
| Abstract: | "This study evaluated the proliferation effects of huangqi on neuron regeneration. We investigated the molecular mechanisms, which include: (1) cyclin D1, A, E-cell cycle factors and MAPK signaling proliferation (2) FGF-2-UPA-MMPs migration signaling. After treatment with various Huanqi concentrations (1.25, 12.5, 125, 250 and 500 μg/ml,), we observed that Huanqi can increase Rsc 96 cell proliferation at 12.5 μg/ml (p < 0.01) concentration determined by the MTT and wound healing tests. Examination by RT-PCR and Western blotting assay showed that Huangqi is able to stimulate the mRNA and protein expressions of cyclin D1, A, E, cell cycle controlling proteins and excite ERK and P38 MAPK signaling pathways to promote cell proliferation. Huangqi stimulates the FGF-2-UPA-MMP 9 migration pathway and enhances RSC 96 Schwann cells migration. Using MAPK chemical inhibitors, U0126, SB203580 and SP600125, the proliferative effects of Huangqi on RSC 96 cells were ERK and P38 signaling-dependent. Based on these results, applying an appropriate dose of Huangqi with biomedical materials would be a potential approach to enhancing neuron regeneration.[ABSTRACT FROM AUTHOR]
Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.Copyright applies to all Abstracts." |
| Relation: | AMERICAN JOURNAL OF CHINESE MEDICINE |
| Appears in Collections: | [生物科技學系] 期刊論文
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