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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/16153

    Title: Dangshen (Codonopsis pilosula) Activates IGF-I and FGF-2 Pathways to Induce Proliferation and Migration Effectsin RSC96 Schwann Cells
    Authors: ;Chen, Hsien-Tung;Tsai, Ying-Lan;Chen, Yueh-Sheng;Jong, Guo-Ping;Chen, Wei-Kung;Wang, Hwai-Lee;Tsai, Fuu-Jen;Tsai, Chang-Hai;Lai, Tung-Yuan;Tzang, Bor-Show;黃志揚;HUANG, CHIH-YANG;Lu, Chung-Yen
    Contributors: 生物科技學系
    Date: 2010
    Issue Date: 2012-11-23 17:09:14 (UTC+8)
    Abstract: This study evaluates the proliferative and migrative effects of dangshen on RSC96, Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and MAPK pathways. (2) migrate and anti-scar signaling, FGF-2-uPA-MMPs. After treatment with different concentrations (20 microg/ml, 40 microg/ml, 60 microg/ml, 80 microg/ml, and 100 microg/ml) of dangshen. We observed a dose dependent proliferative effect using PCNA Western blotting assay, MTT assay and the wound healing test. We also found that dangshen stimulates the protein expressions of IGF-I pathway regulators, cell cycle controlling proteins and excites the MAPK signaling pathway regulators ERK and P38. Dangshen even stimulates the FGF-2-uPA-MMP 9 migration pathway in RSC 96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of dangshen on RSC 96 cells were identified to be ERK- and P38- dependent. Based on these results, applying an appropriate dose of dangshen with biomedical materials would be a potential approach for enhancing neuron regeneration.
    Relation: AMERICAN JOURNAL OF CHINESE MEDICINE, 38(2):359-372.
    Appears in Collections:[生物科技學系] 期刊論文

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