English  |  正體中文  |  简体中文  |  Items with full text/Total items : 93288/109022 (86%)
Visitors : 579126      Online Users : 404
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version

    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/16167

    Title: Rapid detection of two-protein interaction with a single fluorophore by using a microfuidic device.
    Authors: ;Chou, CK;Jing, N;Yamaguchi, H;Tsou, PH;Lee, HH;Chen, CT;Wang, YN;Hong, S;Su, C;Kameoka, J;洪明奇;Hung, Mien-Chie
    Contributors: 生物科技學系
    Date: 2010
    Issue Date: 2012-11-23 17:09:25 (UTC+8)
    Abstract: We have developed a microfluidics based platform and methodology named MAPS (microfluidic system for analyzing proteins in single complex) for detecting two protein interactions rapidly using a single fluorophore. Target proteins were labelled with Quantum dot 525 (QD525) via specific polyclonal antibodies, and were transported through the microfluidic channel subsequently, where the 375 nm excitation laser light was focused to form a detection volume. Photon bursts from target proteins passing through the detection volume were recorded and their photon burst histograms were plotted which demonstrated roughly the specific protein interaction ratio based on their population and statistical behavior. As a proof of concept, Src/STAT3 protein complex interaction ratios with and without EGF stimulation were obtained by MAPS within 1 h and the results were well matched with the one obtained by the conventional immunoprecipitation/Western blot (IP/WB).
    Relation: ANALYST
    Appears in Collections:[生物科技學系] 期刊論文

    Files in This Item:

    File Description SizeFormat

    All items in ASIAIR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback