English  |  正體中文  |  简体中文  |  Items with full text/Total items : 92472/107804 (86%)
Visitors : 19193601      Online Users : 286
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version

    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/16267

    Title: Japanese Encephalitis Virus Envelope Protein Mitigates TNF-! mRNA Expression in RAW264.7 Cells
    Authors: ;Hsu, Tsai-Ching;Gao, Ji-Qiang;Lu, Ko-Hsiu;Tsai, Chang-Hai;黃志揚;HUANG, CHIH-YANG;Tzang, Bor-Show
    Contributors: ç”Ÿç‰©ç§‘技學系
    Keywords: Japanese encephalitis virus;monocyte;envelope protein;TNF-α;p21Waf1/Cip1
    Date: 2008-04
    Issue Date: 2012-11-23 17:10:43 (UTC+8)
    Abstract: Japanese encephalitis virus (JEV) is known as an important mosquito-borne human pathogen that causes Japanese encephalitis and may lead to lethal effect. Since monocyte has been demonstrated to play transmissible role for JEV, rare study is reported to clarify the effect of JEV envelope (JEVE) protein on monocyte. This study intends to investigate the effects of JEVE protein inside monocyte. Notably, significant decreased expression of tumour necrosis factor (TNF)-α mRNA in RAW264.7 cells transfected with pEGFP-JEVE was observed as compared to those cells transfected with pEGFP. Increased p21Waf1/Cip1 protein was observed in both pEGFP and pEGFP-JEVE transfected RAW264.7 cells. However, increased p53 protein was only detected in pEGFP-transfected cells but not pEGFP-JEVE transfected cells as well as the result that no increased expression of nuclear factor-kB was observed in pEGFP-JEVE transfected cells. These experimental results indicate the effects of JEVE protein in alleviating TNF-α mRNA expression that is associated with the increased p53-independent p21Waf1/Cip1 expression and provide an explanation in the role of JEV transmission through monocyte.
    Relation: INFLAMMATION
    Appears in Collections:[生物科技學系] 期刊論文

    Files in This Item:

    File Description SizeFormat

    All items in ASIAIR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback