Please use this identifier to cite or link to this item:
|Title: ||Cardiac Fasdependent and mitochondriadependent apoptosis in ovariectomized rats|
|Authors: ||;Lee, Shin-Da;Kuo, Wei-Wen;Ho, Ying-Jui;Lin, Ann-Chi;Tsai, Chang-Hai;Wang, Hsueh-Fang;Kuo, Chia-Hua;Yang, Ai-Lun;黃志揚;HUANG, CHIH-YANG;Hwang, Jin-Ming|
|Keywords: ||"Heart;Type I apoptosis;Type II apoptosis;Menopause;Ovariectomy;Oophorectomy;Caspase;"|
|Issue Date: ||2012-11-23 17:10:56 (UTC+8)|
|Abstract: ||"Background: Very limited information has been published regarding cardiac apoptosis in menopausal
women or in those after bilateral oophorectomy. The purpose of this study was to evaluate whether
cardiac Fas-dependent (type I) and mitochondria-dependent (type II) apoptotic pathways are activated in
Methods: Thirty-two female Wistar rats at 6–7 months of age were randomly divided into sham-operated
group (Sham) and bilateral ovariectomized group (OVX). Two months after the operation, the cardiac characteristics, myocardial architecture, and two major apoptotic pathways in the excised left ventricle from
rats were measured by histopathological analysis, Western blotting and reverse transcription polymerase
chain reaction (RT-PCR), and positive TUNEL assays.
Results: The whole heart weight, the left ventricular weight, the ratios of whole heart weight to tibia
length, and the ratios of left ventricle to tibia length were signiﬁcantly increased in OVX relative to Sham.
Abnormal myocardial architecture, enlarged interstitial spaces, more minor cardiac ﬁbrosis, and more
cardiac TUNEL-positive apoptotic cells were observed in OVX. The key components of Fas-dependent
apoptosis (TNF-alpha, Fas ligand, Fas death receptors, Fas-associated death domain (FADD), activated
caspase 8, and activated caspase 3) and key components of mitochondria-dependent apoptosis (Bad, Bax,
Bax-to-Bcl2 ratio, cytosolic cytochrome c, activated caspase 9, and activated caspase 3) were signiﬁcantly
increased in OVX hearts.
Conclusions: The absence of female ovaries will activate the cardiac Fas-dependent and mitochondriadependent apoptotic pathways, which might provide one of possible mechanism for developing heart
failure in post-menopause women."
|Appears in Collections:||[生物科技學系] 期刊論文|
Files in This Item:
All items in ASIAIR are protected by copyright, with all rights reserved.