Cardiac Fasdependent and mitochondriadependent apoptosis in ovariectomized rats

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/16284

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Title:	Cardiac Fasdependent and mitochondriadependent apoptosis in ovariectomized rats
Authors:	;Lee, Shin-Da;Kuo, Wei-Wen;Ho, Ying-Jui;Lin, Ann-Chi;Tsai, Chang-Hai;Wang, Hsueh-Fang;Kuo, Chia-Hua;Yang, Ai-Lun;黃志揚;HUANG, CHIH-YANG;Hwang, Jin-Ming
Contributors:	生物科技學系
Keywords:	"Heart;Type I apoptosis;Type II apoptosis;Menopause;Ovariectomy;Oophorectomy;Caspase;"
Date:	2008-10
Issue Date:	2012-11-23 17:10:56 (UTC+8)
Abstract:	"Background: Very limited information has been published regarding cardiac apoptosis in menopausal women or in those after bilateral oophorectomy. The purpose of this study was to evaluate whether cardiac Fas-dependent (type I) and mitochondria-dependent (type II) apoptotic pathways are activated in ovariectomized rats. Methods: Thirty-two female Wistar rats at 6–7 months of age were randomly divided into sham-operated group (Sham) and bilateral ovariectomized group (OVX). Two months after the operation, the cardiac characteristics, myocardial architecture, and two major apoptotic pathways in the excised left ventricle from rats were measured by histopathological analysis, Western blotting and reverse transcription polymerase chain reaction (RT-PCR), and positive TUNEL assays. Results: The whole heart weight, the left ventricular weight, the ratios of whole heart weight to tibia length, and the ratios of left ventricle to tibia length were signiﬁcantly increased in OVX relative to Sham. Abnormal myocardial architecture, enlarged interstitial spaces, more minor cardiac ﬁbrosis, and more cardiac TUNEL-positive apoptotic cells were observed in OVX. The key components of Fas-dependent apoptosis (TNF-alpha, Fas ligand, Fas death receptors, Fas-associated death domain (FADD), activated caspase 8, and activated caspase 3) and key components of mitochondria-dependent apoptosis (Bad, Bax, Bax-to-Bcl2 ratio, cytosolic cytochrome c, activated caspase 9, and activated caspase 3) were signiﬁcantly increased in OVX hearts. Conclusions: The absence of female ovaries will activate the cardiac Fas-dependent and mitochondriadependent apoptotic pathways, which might provide one of possible mechanism for developing heart failure in post-menopause women."
Relation:	MATURITAS
Appears in Collections:	[生物科技學系] 期刊論文

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