English  |  正體中文  |  简体中文  |  Items with full text/Total items : 90120/105278 (86%)
Visitors : 9137087      Online Users : 1026
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/16328


    Title: Proliferation- and migration- enhancing effects of Ginseng and Ginsenoside Rg1 through IGF-I- and FGF-2-signaling pathways on RSC96 Schwann Cells
    Authors: ;Lu, Ming-Chin;Lai, Tung-Yuan;Hwang, Jin-Ming;Chen, Hsien-Te;Chang, Sheng-Huang;Tsai, Fuu-Jen;Wang, Hwai-Lee;Lin, Chien-Chung;Kuo, Wei-Wen;黃志揚;HUANG, CHIH-YANG
    Contributors: 生物科技學系
    Keywords: ginseng;Rg1;proliferate and migrate effect;Schwann cells;peripheral nerve regeneration
    Date: 2009-06
    Issue Date: 2012-11-23 17:11:33 (UTC+8)
    Abstract: The aim of the present study is to evaluate the proliferation- and migration-enhancing effects of ginseng and its component, ginsenoside (Rg1) on RSC96 Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and mitogen-activated protein kinase (MAPK) pathways, (2) migrating and anti-scar signaling, FGF-2-uPA-MMPs.We treated RSC96 cells with different concentrations (100, 200, 300, 400, 500 µg ml−1) of ginseng and its constituent, Rg1 (5, 10, 15, 20, 25 µg ml−1). We observed a proliferative effect in a dose-dependent manner by PCNA western blotting assay, MTT assay, and wound healing test. Furthermore, we also found in the results of western blotting assay, ginseng and Rg1 enhance protein expression of IGF-I pathway regulators, cell cycle controlling proteins, and MAPK signaling pathways to promote the cell proliferation. In addition, ginseng and Rg1 also stimulated the FGF-2-uPA-MMP 9 migrating pathway to enhance the migration of RSC96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of ginseng and Rg1 on RSC96 cells were identified to be MAPK signaling-dependent. On the basis of the results, applying appropriate doses of ginseng and Rg1 with biomedical materials would be a potential approach for enhancing neuron regeneration. Copyright © 2009 John Wiley & Sons, Ltd.
    Relation: CELL BIOCHEMISTRY AND FUNCTION
    Appears in Collections:[生物科技學系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML153View/Open


    All items in ASIAIR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback