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    Title: Curcumin-induced DNA damage and inhibited DNA repair genes expressions in mouse-rat hybrid retina ganglion cells (N18)
    Authors: 呂旭峰;Lu, HF;楊家欣;Yang, JS;賴光啟;Lai, KC;徐素琴;Hsu, SC;Hsueh, SC;Chen, YL;江若華;Chiang, JH;呂啟誠;Lu, CC;羅琦;Lo, C;楊美都;Yang, MD;鍾景光;Chung, Jing-Gung
    Contributors: 生物科技學系
    Keywords: Curcumin;DNA damage;DNA repair;N18 mouse–rat hybrid retina ganglion cells
    Date: 2009-08
    Issue Date: 2012-11-23 17:11:42 (UTC+8)
    Abstract: Curcumin is reported to be a potent inhibitor of the initiation and promotion of many cancer cells. We investigated to examine whether or not curcumin induce DNA damage in mouse–rat hybrid retina ganglion cell line N18 cells. The Comet assay showed that incubation of N18 cells with 10, 25 and 30 μM of curcumin led to a longer DNA migration smear (Comet tail). The DNA gel electrophoresis showed that 20 μM of curcumin for 24 and 48 h treatment induced DNA damage and fragments in N18 cells. The real time PCR analysis showed that 20 μM of curcumin for 48 h treatment decreased ATM, ATR, BRCA1, 14-3-3σ, DNA-PK and MGMT mRNA, and ATM and MGMT mRNA expression were inhibited in a time-dependent manner. Our results indicate that curcumin caused DNA damage and inhibited DNA repair genes which may be the factors for curcumin-inhibited cell growth.
    Relation: NEUROCHEMICAL RESEARCH, 34(8):1491-1497.
    Appears in Collections:[Department of Biotechnology] Journal Article

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