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    题名: n-Butylidenephthalide induced apoptosis in the A549 human lung adenocarcinoma cell line by coupled down-regulation of AP-2α and telomerase activity
    作者: 余永倫;Yu, Yung-luen;Wei, Chyou-wei;Lin, Chai-ching;Lin, Chai-yi;Lin, Po-cheng;Wu, Min-tze;Chen, Cheng-jueng;Chang, Wenliang;Lin, Shinn-zong;Chen, Yi-lin Sophia;Ha, Horng-jyh
    贡献者: 生物科技學系
    关键词: human telomerase reverse transcriptase;n-butylidenephthalide;AP-2 xenograft;immunohistochemistry
    日期: 2009-09
    上传时间: 2012-11-23 17:11:52 (UTC+8)
    摘要: AIM:
    To investigate the role of hTERT gene expression and AP-2alpha in n-butylidenephthalide (n-BP)-induced apoptosis in A549 lung cancer cells.
    Viability of A549 cells was measured by MTT assay. Protein expression was determined by Western blot. Telomerase activity was measured using the modified telomere repeat amplification protocol (TRAP) assay. Xenograft mice were used as a model system to study the cytotoxic effect of n-BP in vivo. The morphology of tumor was examined by immunohistochemical staining.
    The growth of A549 lung cancer cells treated with n-BP was significantly inhibited. Telomerase activity and hTERT mRNA expression were determined by telomeric repeat amplification protocol and reverse transcription-polymerase chain reaction, respectively. n-BP inhibited telomerase activity and hTERT mRNA expression in A549 cells while overexpression of hTERT could abolish BP-induced growth inhibition in the A549 cells. We also showed that hTERT promoter activity in the presence of n-BP was mediated via AP-2alpha. We saw an inhibition of tumor growth when nude mice carrying A549 subcutaneous xenograft tumors were treated with n-BP. Immunohistochemistry of this tumor tissue also showed a decrease in the expression of hTERT.
    The antiproliferative effects of n-BP on A549 cells in vitro and in vivo suggest a novel clinical application of this compound in the treatment of lung cancers.
    關聯: ACTA PHARMACOLOGICA SINICA;30(9):1297-306.
    显示于类别:[生物科技學系] 期刊論文


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