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    Title: Proteomics-based identification of haptoglobin as a novel plasma biomarker in oral squamous cell carcinoma
    Authors: 林振文;LIN, CHENG-WEN;黃素華;Huang, Su-Hua
    Contributors: 生物科技學系
    Keywords: Oral squamous cell carcinoma;Biomarkers;Haptoglobin;Proteomic analysis
    Date: 2010-03
    Issue Date: 2012-11-23 17:12:45 (UTC+8)
    Abstract: "Background
    Identification of tumor biomarkers to assist early diagnosis and monitoring of disease progression may potentially decrease the mortality and morbidity associated with oral cancer.

    A mouse model with oral squamous cell carcinoma (OSCC) induced by 4-nitroquinoline 1-oxide (4-NQO)/arecoline in drinking water was established to discover stage-associated biomarkers. A proteomics approach, immunoblot and immunohistochemical analysis were used to validate the expressed biomarkers in mice with OSCC. Human plasma samples were also collected and candidate biomarkers were evaluated using enzyme-linked immunosorbent assay.

    Proteomic profiling of mouse plasma samples indicated that haptoglobin and apolipoprotein A1 precursor were up-regulated in the mice with OSCC. Immunoblotting of plasma samples and immunohistochemical analysis of oral tissues showed a significantly higher level of haptoglobin in the OSCC mice than in the control mice. The expression of haptoglobin in human plasma samples from 52 patients with OSCC indicated a strong correlation between the increasing levels of haptoglobin and the clinical stages of OSCC (P < 0.01).

    These results suggest that haptoglobin has a great potential as a sensitive plasma biomarker for early detection of patients with OSCC.

    2-DE, 2-dimentional gel electrophoresis; 4-NQO, 4-nitroquinoline 1-oxide; OSCC, oral squamous cell carcinoma; Q-TOF, quadrupole-time of flight"
    Appears in Collections:[Department of Biotechnology] Journal Article

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