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    题名: E4BP4 is a cardiac survival factor and essential for embryonic heart development
    作者: ;Weng, Yi-Jiun;Hsieh, Dennis Jine-Yuan;Kuo, Wei-Wen;Lai, Tung-Yuan;Hsu, Hsi-Hsien;Tsai, Chang-Hai;Tsai, Fuu-Jen;Lin, Ding-Yu;James, A.Lin;黃志揚;HUANG, CHIH-YANG;Tung, Kwong-Chung
    贡献者: 生物科技學系
    关键词: E4BP4;Heart development;Cardiac survival factor
    日期: 2010-06
    上传时间: 2012-11-23 17:13:11 (UTC+8)
    摘要: The bZIP transcription factor E4BP4, has been demonstrated to be a survival factor in pro-B lymphocytes. GATA factors play important roles in transducing the IL-3 survival signal and transactivating the downstream survival gene, E4BP4. In heart, GATA sites are essential for proper transcription of several cardiac genes, and GATA-4 is a mediator of cardiomyocyte survival. However, the role E4BP4 plays in heart is still poorly understood. In this study, Dot-blot hybridization assays using Dig-labeled RNA probes revealed that the E4BP4 gene was expressed in cardiac tissue from several species including, monkey, dog, rabbit, and human. Western blot analysis showed that the E4BP4 protein was consistently present in all of these four species. Furthermore, immunohistochemistry revealed that the E4BP4 protein was overexpressed in diseased heart tissue in comparison with normal heart tissue. In addition, the overexpression of E4BP4 in vitro activated cell survival signaling pathway of cardiomyocytes. At last, siRNA-mediated knock down of E4BP4 in zebrafish resulted in malformed looping of the embryonic heart tube and decreased heart beating. Based on these results, we conclude that E4BP4 plays as a survival factor in heart and E4BP4 is essential for proper embryonic heart development.
    显示于类别:[生物科技學系] 期刊論文




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