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http://asiair.asia.edu.tw/ir/handle/310904400/16505
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Title: | Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways |
Authors: | ;KUO, JEHN-HWA;朱永麟;CHU, YUNG-LIN;楊家欣;YANG, JAI-SING;林景彬;LIN, JING-PIN;賴光啟;LAI, KUANG-CHI;郭秀滿;KUO, HSIU-MAAN;夏德椿;HSIA, TE-CHUN;鍾景光;Chung, Jing-Gung |
Contributors: | 生物科技學系 |
Keywords: | "cantharidin;human bladder cancer TSGH 8301 cells;apoptosis;mitochondria" |
Date: | 2010-11 |
Issue Date: | 2012-11-23 17:13:58 (UTC+8) |
Abstract: | "Cantharidin has shown potent anticancer activities
on many types of human cancer cells. This study was
performed to elucidate whether mitochondria and caspases
are involved in the modulation of apoptosis and cell cycle
arrest by cantharidin in human bladder cancer cells. The
effect of cantharidin on cell cycle arrest, apoptosis, caspases,
reactive oxygen species (ROS) and mitochondrial membrane
potential (Δæm) were measured by flow cytometry, and the
levels of apoptosis-associated proteins and its regulatory
molecules were studied by Western blotting. Cantharidininduced
apoptosis and DNA damage was determined by
flow cytometric analysis, DAPI staining and Comet assay.
After cantharidin treatment, the active forms of caspase-3, -8
and -9 were promoted. Cantharidin-induced apoptosis was
associated with enhanced ROS and Ca2+ generations, caused
DNA damage, decreased the levels of Δæm and promoted
Endo G and AIF released from mitochondria. Cantharidininduced
G0/G1 arrest was associated with a marked decrease
in the protein expressions of cyclin E and Cdc25c but
promoted the levels of p21 and p-p53. Cantharidin-induced
apoptosis was accompanied with up-regulation of the protein
expression of Bax and PARP, but down-regulation of the
protein levels of Bcl-2, resulting in dysfunction of mitochondria
then led to Endo G and AIF release for causing
induction of apoptosis." |
Relation: | INTERNATIONAL JOURNAL OF ONCOLOGY |
Appears in Collections: | [生物科技學系] 期刊論文
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