Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/16505

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Title:	Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways
Authors:	;KUO, JEHN-HWA;朱永麟;CHU, YUNG-LIN;楊家欣;YANG, JAI-SING;林景彬;LIN, JING-PIN;賴光啟;LAI, KUANG-CHI;郭秀滿;KUO, HSIU-MAAN;夏德椿;HSIA, TE-CHUN;鍾景光;Chung, Jing-Gung
Contributors:	生物科技學系
Keywords:	"cantharidin;human bladder cancer TSGH 8301 cells;apoptosis;mitochondria"
Date:	2010-11
Issue Date:	2012-11-23 17:13:58 (UTC+8)
Abstract:	"Cantharidin has shown potent anticancer activities on many types of human cancer cells. This study was performed to elucidate whether mitochondria and caspases are involved in the modulation of apoptosis and cell cycle arrest by cantharidin in human bladder cancer cells. The effect of cantharidin on cell cycle arrest, apoptosis, caspases, reactive oxygen species (ROS) and mitochondrial membrane potential (Δæm) were measured by flow cytometry, and the levels of apoptosis-associated proteins and its regulatory molecules were studied by Western blotting. Cantharidininduced apoptosis and DNA damage was determined by flow cytometric analysis, DAPI staining and Comet assay. After cantharidin treatment, the active forms of caspase-3, -8 and -9 were promoted. Cantharidin-induced apoptosis was associated with enhanced ROS and Ca2+ generations, caused DNA damage, decreased the levels of Δæm and promoted Endo G and AIF released from mitochondria. Cantharidininduced G0/G1 arrest was associated with a marked decrease in the protein expressions of cyclin E and Cdc25c but promoted the levels of p21 and p-p53. Cantharidin-induced apoptosis was accompanied with up-regulation of the protein expression of Bax and PARP, but down-regulation of the protein levels of Bcl-2, resulting in dysfunction of mitochondria then led to Endo G and AIF release for causing induction of apoptosis."
Relation:	INTERNATIONAL JOURNAL OF ONCOLOGY
Appears in Collections:	[生物科技學系] 期刊論文

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