English  |  正體中文  |  简体中文  |  Items with full text/Total items : 90453/105672 (86%)
Visitors : 12224615      Online Users : 675
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/16705


    Title: Characterization of the Charge Variants of L2 β-lactamase in Stenotrophomonas maltophilia
    Authors: 胡若梅;Hu, Rouh-Mei;Chiang, Kai-Hung;Chang, Yi-Chih;Yang, Tsuey-Ching
    Contributors: 生物與醫學資訊學系
    Date: 2009-03
    Issue Date: 2012-11-23 17:16:06 (UTC+8)
    Abstract: Stenotrophomonas maltophilia KH has two acid b-lactamases with isoelectric points (pIs) of 4.6 and 5.4, and several basic b-lactamases (pIs .7.0) that produce a ladder-shaped pattern by IEF.
    An isogenic L2 mutant, KHL2xylE, was constructed by gene replacement. From IEF and native PAGE zymograms of strains KH and KHL2xylE, it was demonstrated that the basic b-lactamases and the acid b-lactamase with pI 5.4 are encoded by the same L2 gene and that the active types of these L2 charge variants were dependent on the buffer pH. The b-lactamase activities of these L2 charge variants in phosphate buffer at pH 7.0 and 8.0 were 1075±29 and 1114±81 U mg”1 , respectively. These results indicate that L2 charge variants give S. maltophilia a better chance of adapting and surviving in response to changes in the environment.
    Relation: JOURNAL OF MEDICAL MICROBIOLOGY,58(3),318-321.
    Appears in Collections:[生物資訊與醫學工程學系 ] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML160View/Open


    All items in ASIAIR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback