"Butylated hydroxyanisole (BHA), a synthetic
antioxidant, has been used in fat and fatty foods to prevent
oxidative deterioration. However, the functions of BHA on
immune responses in normal mice remain elusive. The aim of
the present study was to investigate the effects of oral treatment of BHA on immune responses in normal mice in vivo.
BALB/c mice received various treatments. Blood samples were
collected and analyzed. Flow cytometry was used to determine
the levels of the cell markers. Results showed that BHA did not
significantly affect the weight of the animal body and spleen in
normal mice. BHA promoted macrophage phagocytosis from
peripheral blood mononuclear cells, but did not alter this process
in the peritoneal cavity. Furthermore, BHA did not influence
natural-killer cell cytotoxicity in normal mice. Notably, BHA
promoted the levels of CD3 (T cells) and decreased the level
of CD19 (B cells), but did not significantly affect the levels of
CD11b (monocytes) and macrophages (Mac-3) in normal mice.
Based on these observations it can be concluded that BHA
promotes immune responses by increasing T cells and activating phagocytosis by macrophages in normal mice. However,
the molecular mechanisms require further investigation."