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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/16883


    Title: Fisetin and rutin as 3C protease inhibitors of enterovirus A71
    Authors: 林振文;LIN, CHENG-WEN
    Contributors: 生物科技學系
    Keywords: Enterovirus A71;3C protease;Flavonoids;Fisetin;Rutin
    Date: 2012-06
    Issue Date: 2012-11-23 17:18:03 (UTC+8)
    Abstract: Enterovirus A71 (EV-A71) causes severe complications: encephalitis, pulmonary edema, and death. No effective drug has been approved for clinical use. This study investigated the antiviral effects of flavonoids against EV-A71. An in vitro inhibitor screening assay using recombinant EV-A71 3C protease (3Cpro) demonstrated fisetin and rutin inhibiting 3Cpro enzymatic activity in a dose-dependent manner. Cell-based fluorescence resonance energy transfer (FRET) assay with an EV-A71 3Cpro cleavage motif probe also confirmed that fisetin and rutin inhibited the replication of EV-A71 in cells. A virus replication assay indicated that fisetin and rutin reduced significantly the EV-A71-induced cytopathic effect and viral plaque titers in RD cells culture. The IC50 values of plaque reduction against EV-A71 were 85 μM for fisetin and 110 μM for rutin. Therapeutic indices (CC50/IC50 of plaque reduction assays) of fisetin and rutin exceeded 10. The study suggests that fisetin and rutin inhibit the replication of EV-A71.
    Relation: JOURNAL OF VIROLOGICAL METHODS, V.182 N.1-2:93-98.
    Appears in Collections:[生物科技學系] 期刊論文

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