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    题名: Identification of six common species of processed filefish using cytochrome b gene sequence and PCR-RFLP analysis
    作者: 謝承紘;Hsieh, Cheng-Hong
    贡献者: 保健營養生技學系
    日期: 2008-12
    上传时间: 2012-11-26 10:32:49 (UTC+8)
    摘要: Sequence analysis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique were used to identify the commercial species of Aluterus monoceros, Aluterus scriptus, Monacanthus chinensis, Thamnaconus hypargyreus, Thamnaconus modestus and Chaetodermis penicilligerus. The primers L14735 and H15149ad were designed in the mitochondrial cytochrome b gene and the molecular weight of amplifi ed fragment from processed fi lefi sh meats was 465 bp. Furthermore, the results obtained from the HaeIII enzyme digestion could be used to distinguish the six commercial fi lefi sh species in fresh and heat-treated meats. Using the PCR-RFLP method, six fi lefi sh species could be successfully identifi ed in fresh and heat-treated meats. The PCR-RFLP technique developed in this study was proved to be a rapid, reliable and simple method that enables easy and accurate identifi cation of six commercial fi lefi sh processed species.
    显示于类别:[食品營養與保健生技學系] 期刊論文


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