Objectives: The purpose of this study is to investigate the effect of health education on the prevention of Norovirus infection in the seventh grade of middle school students regarding knowledge of Norovirus, steps (and timing) of hand washing, and personal protective measures.Methods: This study used a quasi-experimental design. The study population is all seventh grade middle school students in Yunlin County. Four hundred seventy-four students were sampled using a multi-stage cluster sampling method. They were further divided into health education group (221 students) and control group (253 students). Questionnaires were administered to students in both groups before and after the start of the health education program. Only students in health education group received health education on the prevention of Norovirus infection. A post-test was conducted one week after the education program in order to assess the effect of intervention. Contents of the questionnaires include basic and demographic information of students, questions regarding knowledge about Norovirus, measuring scale of the 5 steps of correct hand washing, and measuring scales of the timing of hand washing and personal protective measures.Results: Knowledge toward the prevention of Norovirus infection improved significantly in the health education group. The proportion of accuracy rate increased from 25.8% to 63.3%. Test scores improved after intervention in the health education group in the steps of hand washing, knowledge toward prevention of Norovirus infection, personal protective behaviors, and timing of hand washing respectively.Conclusion: Health education is effectively improving knowledge of prevention of Norovirus infection and the correct steps of hand washing in the seventh grade of middle high school students. We suggest continued promotion of health education on the prevention of Norovirus infection and hand washing, for strengthening the attitude of students toward hand washing and reducing the occurrence of gastrointestinal diseases.