This study focused on the investigation of the possibility of monitoring blood glucose levels in
humans by non-invasively extracting glucose and lactate from blood through the skin using reverse
iontophoresis. In vitro reverse iontophoresis studies have indicated that the optimum switching mode
for reverse iontophoresis of lactate and glucose are continuous direct current and direct current with
electrode polarity reversal every 15 minutes, respectively. The application of a current combined with
electrode polarity reversal every 15 minutes has been suggested for use in humans. The reverse
iontophoresis technique was applied to 10 healthy volunteers. Glucose and lactate were successfully
extracted through the subjects’ skin into the methylcellulose gel of the electrodes. A fair-good
correlation (r2 = 0.62) between the subject’s blood glucose level and the ratio of glucose to lactate
levels in the collection gels was observed after two outliers were removed from the regression equation.
The result suggests that it may be possible to non-invasively monitor the blood glucose levels using this
new approach free of the need for calibration with a blood sample.
Asian Journal of Health and Information Sciences 1(4):393-410