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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/2459


    Title: Study of gene transfer using pollen electrotransformation method in Easter lily(Lilium longiflorum Thunb.)
    Authors: Lee Kung Chge
    Contributors: Department of Biotechnology
    Keywords: lily;pollen;pollen electrotransformation
    Date: 2005
    Issue Date: 2009-11-06 14:09:31 (UTC+8)
    Publisher: Asia University
    Abstract: Easter lily is one of the important floral crops in the world, but the pity is that of less variation in flower color except the white clor is noted. The purpose of this study was to evaluate the possibility of flower color gene transformation in Easter lily using pollen electrotransformation method. Pollen germination rate was the highest with the best activity at first blooming day, and decreased with following days after anthesis. The germination rate was up to 80% in special liquid medium supplemented with 6% sucrose, 40mg/L boric acid and 40mg/L CaCl2 at 25~30C. The stigma vigor has a better activity and with higher fruit setting at first blooming day to 5 days after flowering. Sitgma with 3/4 style cut-off was formol to have better effect in liquid pollination process. Young leaves were cultured on medium supplemented with 50mg/L hygromycine for selecting transformed plantlets. According to this pollen electrotransformation method, dfr gene was transferred into germinating pollen, then pollinated onto the normal flowers. After pollination, seeds were gathered and planted. Genomic DNA was extracted from the leaves of putative transformats. Results form PCR and DIG-labelled Southern assays indicated that dfr gene were transferred into transgenic progenies, this suggested that foreign gene could be transformed into Easter lily by means of pollen electrotransformation method.
    Appears in Collections:[生物科技學系] 博碩士論文

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