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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/2475


    Title: Evaluation of Genetic Diversity among Indigenous Cinnamon(Cinnamomum osmophloeum KANEH.) by RAPD and ISSR Markerst
    Authors: huang jian ye
    Contributors: Department of Biotechnology
    Keywords: Cinnamomum osmophloeum KANEH., RAPD, ISSR, Genetic diversity, cinnamaldehyde
    Date: 2006
    Issue Date: 2009-11-06 14:10:04 (UTC+8)
    Publisher: Asia University
    Abstract: Indigenous cinnamon (Cinnamomum osmophloeum KANEH.)
    (Lauraceae) is a unique specie of camphor tree in Taiwan. The leaves of
    cinnamon are rich in essential oils which can be applied to medicine, food
    and cosmetics.
    The genetic diversity of seventeen accessions collected in Taiwan
    were evaluated by RAPD and ISSR markers.The results indicated that
    both methods revealed high degree of polymorphism and reproduction.
    A totally 103 amplified DNA fragments were generated by 9 selected
    RAPD primers and among them 80 fragments show polymorphic. The
    sizes of DNA fragments were between 3625bp and 250bp. A total 124
    amplified DNA fragments, including 105 polymorphic fragments, were
    generated by 11 selected ISSR primers. The sizes of DNA fragments
    were between 2500 bp and 250 bp. The ratios of polymorphic DNA
    fragments produced by RAPD and ISSR were 77.67% and 84.68%,
    respectively. The similarity between accessions were estimated by using
    the Dice’s formula based on the total amplified and polymorphic DNA
    fragments from RAPD and ISSR. The genetic similarity was analysis by
    UPGMA (Unweighted Pair-Group mean Arithmetic).
    The results showed that UPGMA cluster analysis based on RAPD
    similarity matrixs could separate those varieties to seven distinct groups,
    genetic diversity were between 0.72 and 0.88. The ISSR similarity
    matrixs could separate those varieties to five distinct groups, genetic
    diversity were between 0.66 and 0.85
    Appears in Collections:[生物科技學系] 博碩士論文

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