Please use this identifier to cite or link to this item:
|Title: ||Inhibition of matrix metalloproteinase-9 expression by docosahexaenoic acid mediated by heme oxygenase 1 in 12-O-tetradecanoylphorbol-13-acetate-induced MCF-7 human breast cancer cells|
|Authors: ||Chen, Haw-Wen;Chen, Haw-Wen;趙哲毅;Chao, Che-Yi;Lin, Li-Lin;Lin, Li-Lin;Lu, Chia-Yang;Lu, Chia-Yang;Liu, Kai-Li;Liu, Kai-Li;Chong-Kuei, L;Lii, Chong-Kuei;Chien-Chun, L;Li, Chien-Chun|
|Issue Date: ||2013-07-11 14:02:20 (UTC+8)|
|Abstract: ||Matrix metalloproteinase-9 (MMP-9) plays a crucial role in tumor metastasis. Previous studies showed that polyunsaturated fatty acids exhibit an anti-cancer effect in various human carcinoma cells, but the effect of docosahexaenoic acid (DHA) and linoleic acid (LA) on metastasis of breast cancer cells is not fully clarified. We studied the anti-metastasis potential of DHA and LA in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MCF-7 cells. We found that TPA (100 ng/ml) induced MMP-9 enzyme activity both dose- and time-dependently, and 200 μM DHA and LA significantly inhibited MMP-9 mRNA and protein expression, enzyme activity, cell migration, and invasion. Treatment with PD98059 (10 μM), wortmannin (10 μM), and GF109203X (0.5 μM) decreased TPA-induced MMP-9 protein expression and enzyme activity. TPA-induced activation of ERK1, Akt, and PKCδ was attenuated by DHA, whereas LA attenuated only ERK1 activation. GF109203X also suppressed ERK1 activation. EMSA showed that DHA, LA, PD98059, and wortmannin decreased TPA-induced NF-κB and AP-1 DNA-binding activity. Furthermore, DHA rather than LA dose-dependently increased HO-1 expression. HO-1 siRNA alleviated the inhibition by DHA of TPA-induced MMP-9 protein expression and enzyme activity in MCF-7 cells, and HO-1 knockdown reversed the DHA inhibition of cell migration. These results suggest that DHA and LA have both similar and divergent signaling pathways in the suppression of TPA-induced MCF-7 metastasis.|
|Relation: ||ARCHIVES OF TOXICOLOGY; 87(5):857-69.|
|Appears in Collections:||[食品營養與保健生技學系] 期刊論文|
Files in This Item:
There are no files associated with this item.
All items in ASIAIR are protected by copyright, with all rights reserved.