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    题名: PCR-RFLP analysis using capillary electrophoresis for species identification of Cyprinidae-related products
    作者: Chen, Chien-;Chen, Chien-Hung;謝承紘;Hsieh, Cheng-Hong;黃登福;Hwang, Deng-Fwu
    贡献者: 保健營養生技學系
    关键词: PCR-RFLP;Capillary electrophoresis;Cyprinidae;DNA degradation;Cytochrome b gene;Mitochondria
    日期: 2013-10
    上传时间: 2013-07-11 14:02:27 (UTC+8)
    摘要: Attempts were made to establish specific polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) profiles of 10 different Cyprinidae fish species using capillary electrophoresis (CE). The primer set CypbL/CypbH was used to amplify a partial mitochondrial cytochrome b gene (331 bp), and the product was subsequently co-digested with endonuclease (NlaIII and MseI). The PCR-RFLP profiles of the 10 Cyprinidae fish species determined using CE were able to successfully differentiate the different fish species. This method was further applied to detect the species used in 24 commercial Cyprinidae-related products. The results identified 4 samples as Cyprinus carpio carpio, 8 samples as Carassius auratus auratus, and 12 samples as Ctenopharyngodon idella. This study shows that performing PCR-RFLP in a CE system is useful for identifying the species of fish from which the processed products originated, and this method can be a potential analytical tool for large and routine food authentication in the future.
    關聯: FOOD CONTROL; 33(2):Pages 477–483.
    显示于类别:[食品營養與保健生技學系] 期刊論文


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