Real time imaging of living cell activation is an increasing demand in disciplines of life science and medicine. Techniques like fluorescent, quantum dot are often used in the measurement of living cell activation, but these techniques have to do much processing procedures in preparing samples. Nowadays, surface plasmon resonance (SPR) technique is also used to develop an image platform for measuring cell activation. The surface plasmon resonance technique has the ability of providing real-time information and non-labelling measurement, which makes it very suitable used in biosening. Conventional SPR sensors detect only the average change of refractive index nearby the detecting point on the sensor surface. In the measurement of living cell, it is unable to understand the changes of individual living cells upon stimuli. This study plans to develop a surface plasmon resonance imaging system; we will use a CCD camera and an objective lens for capturing images from the sensing cells. In the experiments, some immunocytes, like mast cells, basophilic granular cell, eosinophilic granule cells, and macrophages, will be used in the measurements. Besides, confocal microscope is used to observe the stimulation results of cells, and these results will compare with the findings in the SPR measurement. This system may be used to investigate the reactions evoked in the near-membrane fields of living cells, and could be used in immunoassay or provide high-throughput information in clinical diagnosis.