Mechanism studies of Taiwan herbal tea Wedelia chinensis (Osbeck) Merr. on hepatoprotective and immunomodulative effects The aim of this study was undertaken to investigate the effects of Wedelia chinensis (Osbeck) Merr. on hepatoprotection and immunopropection. Wedelia chinensis (Hwang-hua-mih-tsay) is a common ingredient of anti-inflammatory herbal medicines in Taiwan, but the efficacy and mechanisms have never been studied. Results from pilot study showed that Wedelia chinensis hot water extract treatment RAW264.7 cells significantly decreased the nitrite production. In addition, the pilot study showed that Wedelia chinensis ethanol extract have the antioxidative, anti-inflammatory and antimicrobial activities. These results suggest that Wedelia chinensis have the potential function of immunomodulation and hepatoprotection. A high number of people in Taiwan suffer from liver diseases. Chronic liver disease and cirrhosis rank sixth on the top 10 leading causes of death. Inflammation is closely related to the progression of various diseases and tissue damage. The hepatoprotective activity of Wedelia chinensis will test in vitro against CCl4-induced damage in human hepatoma cells (HepG2). In addition, free radical-scavenging activity and ferric-reducing antioxidant parameter were used to measure antioxidant activity of crude extract and isolated compounds. These results suggest that the protective effects of crude extract of Wedelia chinensis against the CCl4-induced hepatotoxicity possibly involve the antioxidant effect of these extracts. Mechanism studies were analysed by Western blot and real-time RT-PCR and flow cytometry as in vitro model. Moreover, the in vivo efficacy and mechanisms of action by oral gavage three dosage of Wedelia chinensis extracts were analyzed in different animal models (normal mice and SCID mice) to determine the hepatoprotective and immunopropective effects. Finally, we will evaluate the health protection ability by the college students as in vivo human model. These results suggest that Taiwan herbal tea Wedelia chinensis (Osbeck) Merr. could modulate cellular immune response. This new finding can be the reference for the development of healthy foods for hepatoprotective and immunomodulative in the future; and also for the application of clinical supplementary treatment. Therefore, this study is important for both basic and clinical applications.