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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/4473

    Title: One-step HPLC purification procedure for porcine brain 90-kDa heat shock protein
    Authors: Chu LFN;Lee WC;Yang PC;Chu R;Mao SJT
    Contributors: Department of Biotechnology
    Date: 1997-7
    Issue Date: 2009-11-26 09:43:20 (UTC+8)
    Publisher: Asia University
    Abstract: The 90-kDa heat shock protein (HSP90) was purified from porcine brain by a novel single-step purification procedure using diethylaminoethyl high-performance liquid chromatography (HPLC). About 4.8 mg of HSP90 was isolated from 25 g wet wt porcine brain tissue. The purified protein possessed a single moiety on one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by silver staining. Western blotting using monoclonal antibody prepared against human HSP90 confirmed its identity as HSP90. These results indicate that small-scale HPLC purification of HSP90 from porcine brain tissue can be readily accomplished, with high yield, using a convenient one-step purification method. The procedure described in this paper represents a significant improvement in current purification methods for the isolation of HSP90 from porcine brain.
    Relation: Protein Expression and Purification 10(2):180-4
    Appears in Collections:[生物科技學系] 期刊論文

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