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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/4620

    Title: Cancer-specific activation of the survivin promoter and its potential use in gene therapy
    Authors: Chen, J-S.;Liu, J-C.;Shen, L.;Rau, K-M.;Kuo, H-P.;Li, Y. M.;Shi, D.;Lee, Y-C.;Chang, K-J.;Mien-Chie Hung
    Date: 2004-11
    Issue Date: 2009-11-27 13:57:06 (UTC+8)
    Publisher: Asia University
    Abstract: Survivin is expressed in many cancers but not in normal adult tissues and is transcriptionally regulated. To test the feasibility of using the survivin promoter to induce cancer-specific transgene expression in lung cancer gene therapy, a vector expressing a luciferase gene driven by the survivin promoter was constructed and evaluated in vitro and in vivo. We found that the survivin promoter was generally more highly activated in cancer cell lines than in normal and immortalized normal cell lines. When delivered intravenously by DNA:liposome complexes, the survivin promoter was more than 200 times more cancer specific than the cytomegalovirus promoter in vivo. To identify lung cancer patients who may benefit from gene therapy with the survivin promoter, we measured survivin protein expression in surgical specimens of 75 non-small-cell lung cancers and 10 normal lung tissues by immunohistochemical staining and found that survivin is expressed in most of the non-small-cell lung cancers tested (81%, 61 of 75) but none of the normal lung tissues. The survivin promoter also induced transgene expression of a mutant Bik in cancer cells, which suppressed the growth of cancer cells in vitro and in vivo. These results indicate that the survivin promoter is a cancer-specific promoter for various cancers and that it may be useful in cancer gene therapy.
    Relation: CANCER GENE THERAPY 11(11):740-747
    Appears in Collections:[生物科技學系] 期刊論文

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