Web-based learning design critically effects learning outcome. A standard design criteria and evaluation scale could evaluate websites accurately. This study conducts two phases of research design. A Delphi technique and questionnaire survey conducted in the first phase, establishes initial design criteria. A questionnaire survey followed by a heuristic evaluation in the second phase redefines design criteria. Users interacted with design criteria via real online testing and website learning evaluation. Scholars and professionals then analyzed evaluation results to verify the design criteria value and effects. Contributions of this study include: (1) developing a standardized evaluation organization and development procedure example; (2) providing effective design criteria and an evaluation scale; (3) establishing a basis to guide and evaluate web-based learning platform design.