ASIA unversity:Item 310904400/64353
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 90453/105671 (86%)
造访人次 : 16036757      在线人数 : 123
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻
    ASIA unversity > 資訊學院 > 資訊工程學系 > 期刊論文 >  Item 310904400/64353


    题名: Genotyping of human neutrophil antigens (HNA) from whole genome sequencing data
    作者: 朱學亭;Chu, Hsueh-Ting;林翰;Lin, Han;曹純惠;Theresa, T.H.Tsao;張春梵;Chang, Chun-Fan;William, W.L;William, W.L.Hsiao;葉慈容;Yeh, Tze-Jung;張清貿;Chang, Ching-Mao;劉燕雯;Liu, Yen-Wenn;王澤毅;Wang, Tse-Yi;楊克鈞;Yang, Ko-Chun;陳祟瑞;Chen, Tsung-Jui;陳仁治;Chen, Jen-Chih;陳光琦;Chen, Kuang-Chi;高成炎;Kao, Cheng-Yan
    贡献者: 資訊工程學系
    日期: 201309
    上传时间: 2013-10-29 17:40:27 (UTC+8)
    摘要: Background: Neutrophil antigens are involved in a variety of clinical conditions including transfusion-related acute lung injury (TRALI) and other transfusion-related diseases. Recently, there are five characterized groups of human neutrophil antigen (HNA) systems, the HNA1 to 5. Characterization of all neutrophil antigens from whole genome sequencing (WGS) data may be accomplished for revealing complete genotyping formats of neutrophil antigens collectively at genome level with molecular variations which may respectively be revealed with available genotyping techniques for neutrophil antigens conventionally. Results: We developed a computing method for the genotyping of human neutrophil antigens. Six samples from two families, available from the 1000 Genomes projects, were used for a HNA typing test. There are 500 ∼ 3000 reads per sample filtered from the adopted human WGS datasets in order for identifying single nucleotide polymorphisms (SNPs) of neutrophil antigens. The visualization of read alignment shows that the yield reads from WGS dataset are enough to cover all of the SNP loci for the antigen system: HNA1, HNA3, HNA4 and HNA5. Consequently, our implemented Bioinformatics tool successfully revealed HNA types on all of the six samples including sequence-based typing (SBT) as well as PCR sequence-specific oligonucleotide probes (SSOP), PCR sequence-specific primers (SSP) and PCR restriction fragment length polymorphism (RFLP) along with parentage possibility. Conclusions: The next-generation sequencing technology strives to deliver affordable and non-biased sequencing results, hence the complete genotyping formats of HNA may be reported collectively from mining the output data of WGS. The study shows the feasibility of HNA genotyping through new WGS technologies. Our proposed algorithmic methodology is implemented in a HNATyping software package with user's guide available to the public at
    關聯: BMC Medical Genomics, 6(1):1-8.
    显示于类别:[資訊工程學系] 期刊論文


    档案 大小格式浏览次数


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈