English  |  正體中文  |  简体中文  |  Items with full text/Total items : 90069/105176 (86%)
Visitors : 6542562      Online Users : 102
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/64378


    Title: CCL2 increases αvβ3 integrin expression and subsequently promotes prostate cancer migration
    Authors: Tien-Huang, L;Lin, Tien-Huang;Liu, Hsin-Ho;Liu, Hsin-Ho;Tsung-Hsun, T;Tsai, Tsung-Hsun;Ch, Chi-Cheng;Chen, Chi-Cheng;Hsie, Teng-Fu;Hsieh, Teng-Fu;Le, Shang-Sen;Lee, Shang-Sen;Lee, Yuan-Ju;Lee, Yuan-Ju;Chen, Wen-Chi;Chen, Wen-Chi;湯智昕;Chih-Hsin, Tang
    Contributors: 生物科技學系
    Keywords: CCL2;CCR2;Integrin;Migration;Prostate cancer
    Date: 201310
    Issue Date: 2013-11-01 09:59:57 (UTC+8)
    Abstract: BACKGROUND:

    Chemokine ligand 2 (CCL2), also known as monocyte chemoattractant protein-1 (MCP-1), belongs to the CC chemokine family which is associated with the disease status and outcomes of cancers. Prostate cancer is the most commonly diagnosed malignancy in men and shows a predilection for metastasis to the bone. However, the effect of CCL2 on human prostate cancer cells is largely unknown. The aim of this study was to examine the role of CCL2 in integrin expression and migratory activity in prostate cancers.

    METHODS:

    Prostate cancer migration was examined using Transwell, wound healing, and invasion assay. The PKCδ and c-Src phosphorylations were examined by using western blotting. The qPCR was used to examine the mRNA expression of integrins. A transient transfection protocol was used to examine AP-1 activity.

    RESULTS:

    Stimulation of prostate cancer cell lines (PC3, DU145, and LNCaP) induced migration and expression of integrin αvβ3. Treatment of cells with αvβ3 antibody or siRNA abolished CCL2-increased cell migration. CCL2-increased migration and integrin expression were diminished by CCR2 but not by CCR4 inhibitors, suggesting that the CCR2 receptor is involved in CCL2-promoted prostate cancer migration. CCL2 activated a signal transduction pathway that includes PKCδ, c-Src, and AP-1. Reagents that inhibit specific components of this pathway each diminished the ability of CCL2 to effect cell migration and integrin expression.

    CONCLUSIONS:

    Interaction between CCL2 and CCR2 enhances migration of prostate cancer cells through an increase in αvβ3 integrin production.

    GENERAL SIGNIFICANCE:

    CCL2 is a critical factor of prostate cancer metastasis.
    Relation: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1830(10):4917-27.
    Appears in Collections:[生物科技學系] 期刊論文

    Files in This Item:

    File SizeFormat
    index.html0KbHTML261View/Open


    All items in ASIAIR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback