ASIA unversity:Item 310904400/65082
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 90437/105768 (86%)
造访人次 : 10940160      在线人数 : 633
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/65082


    题名: Gallic acid provokes DNA damage and suppresses DNA repair gene expression in human prostate cancer PC-3 cells.
    作者: Li, Kuo-Ching;Liu, Kuo-Ching;Heng-Chien, H;Ho, Heng-Chien;Hua, An-Cheng;Huang, An-Cheng;Ji, Bin-Chuan;Ji, Bin-Chuan;Lin, Hui-Yi;Lin, Hui-Yi;闕甫伈;Chueh, Fu-Shin;Yan, Jai-Sing;Yang, Jai-Sing;Lu, Chi-Cheng;Lu, Chi-Cheng;Jo-Hua Chiang,;Menghsiao Meng,;鍾景光;Chung, Jing-Gung;、、
    贡献者: 生物科技學系
    关键词: DNA damage;DNA repair;comet assay;gallic acid;human prostate cancer PC-3 cells
    日期: 2013-10
    上传时间: 2013-12-06 14:43:51 (UTC+8)
    摘要: Our earlier studies have demonstrated that gallic acid (GA) induced cytotoxic effects including induction of apoptosis and DNA damage and inhibited the cell migration and invasion in human cancer cells. However, GA-affected DNA damage and repair gene expressions in human prostate cancer cells are still unclear. In this study, we investigated whether or not GA induces DNA damage and inhibits DNA repair gene expression in a human prostate cancer cell line (PC-3). The results from flow cytometric assay indicated that GA decreased the percentage of viable PC-3 cells in a dose- and time-dependent manner. PC-3 cells after exposure to different doses (50, 100, and 200 μM) of GA and various periods of time (12, 24, and 48 h) led to a longer DNA migration smear (comet tail) occurred based on the single cell gel electrophoresis (comet assay). These observations indicated that GA-induced DNA damage in PC-3 cells, which also confirmed by 4,6-diamidino-2-phenylindole dihydrochloride staining and DNA agarose gel electrophoresis. Alternatively, results from real-time polymerase chain reaction assay also indicated that GA inhibited ataxia telangiectasia mutated, ataxia-telangiectasia and Rad3-related, O⁶-methylguanine-DNA methyltransferase, DNA-dependent serine/threonine protein kinase, and p53 mRNA expressions in PC-3 cells. Taken together, the present study showed that GA caused DNA damage and inhibited DNA repair genes as well as both effects may be the critical factors for GA-inhibited growth of PC-3 cells in vitro.
    Copyright © 2011 Wiley Periodicals, Inc., a Wiley company.
    關聯: ENVIRONMENTAL TOXICOLOGY;28(10):579-87.
    显示于类别:[生物科技學系] 期刊論文

    文件中的档案:

    档案 大小格式浏览次数
    index.html0KbHTML170检视/开启


    在ASIAIR中所有的数据项都受到原著作权保护.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈